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Porcine IgG purification kit

Product code: PPA-FF KIT

PRICE: 300 EUR/KIT

KIT CONTENT
(sufficient reagents for 8 purifications with 15 ml porcine serum/each)

  • Porcine IgG Binding Gel (SepharoseTM fast flow) (Code : PPA-FF) : 5 ml gel column.
    Binding capacity : approx. 20 mg Porcine IgG/ml wet gel.
    Purity : 98% by SDS-PAGE.
    Maximum pressure : 3 bars (43 psi, 0.3 MPa).
    Gel life : approx. 50 cycles with routine regeneration.
  • Porcine IgG Binding Buffer (Code : BBP) 2x concentrated : 1,000 ml.
  • Porcine IgG Elution Buffer (Code : EBP) : 500 ml.
    • all IgG from 15 ml of Porcine serum.
 

INSTRUCTION FOR USE

  1. Preparation of Porcine IgG Binding Buffer (Code : BBP) ready to use : Add 1 volume of distilled water to 1 volume of concentrated Porcine IgG Binding Buffer and have so the Porcine IgG Binding Buffer (Code : BBP) ready to use.
  2. Equilibrate the column with 20 ml of Porcine IgG Binding Buffer (BBP).
  3. Load the sample into the column at a flow rate of 30 ml/hour.
  4. Wash the column with 250 ml of Porcine IgG Binding Buffer (BBP).
  5. Elute the Porcine IgG with 50 ml of Porcine IgG Elution Buffer (Code : EBP).
  6. Assay the elution fractions obtained as described in point 5, using the most appropriate system (SDS-PAGE, immunodiffusion, radioimmunoassay, Elisa...)
REGENERATION OF THE PORCINE IgG BINDING GEL
It is recommended to regenerate the gel after every 5 cycles of use.
  1. Wash the column with 10x volumes of acetic acid 0.5M.
  2. Wash the column with 10x volumes of distilled water.
  3. Equilibrate the column 10x volumes of PBS (50 mM K2HPO4, 150mM NaCl) pH 7.4.
  4. Store the column at 4°C in the presence of NaN3 0.1% (w/v).
  5. For the next use, see INSTRUCTION FOR USE as described above.
If you need sterile materials, the regeneration can be carried out as follows.
STERILE REGENERATION OF THE PORCINE IgG BINDING GEL
(GEL SANITIZATION)
AFTER EVERY 5 CYCLES OF USE
  1. Wash the column with 5 volumes of acetic acid 1 M.
  2. Wash the column with 10 volumes of sterile distilled water.
  3. Wash the column with 5 volumes of NaOH 1M.
  4. Wash the column with 10 volumes of sterile distilled water.
  5. Wash the column with 10 volumes of PBS (50 mM K2HPO4, 150mM NaCl) pH 7.4; NaN3 0.1%(w/v).
  6. The sterile gel column is now ready to be re-used.

  IgG, IgM, IgY Purification Kits