CHARACTERISTICS OF THE MATRIX

Matrix: Sepharose^TM CL-4B
and/or Sepharose^TM 4 Fast Flow
Activation method: Oxiran.
Binding capacity: 45 micrograms of Cu²⁺ atom-ion per ml of wet gel.
Stability of the matrix: pH 2-11.
Shelf life of the matrix: Several years, if stored at 4°C in the presence of NaN₃ 0.02% (w/v).
Life time of the matrix with routine use: Approx. 20 cycles with no loss of binding capacity.
Storage: 4°C in PBS pH 7.4; NaN₃ 0.02% (w/v).

ORDERING INFORMATION

Product code	Product name	Package	*Price in EUR
IDA15-4S	Iminodiacetic acid SepharoseTM CL-4B Matrix	100 ml	270.00
		500 ml	930.00
		1000 ml	1600.00
IDA15-4FF	Iminodiacetic acid SepharoseTM 4 Fast Flow Matrix	100 ml	400.00
		500 ml	1400.00
		1000 ml	2400.00

* All prices are FOB Liège.
Bulk quantities are available.

INSTRUCTION FOR USE (Metal Chelating)

1. De-gas the ISA-matrix when using more than 25 ml of matrix.
2. Pour the matrix into a glass column with a frit of porosity P.1.
3. Wash the column with 10 volumes of distilled water.
4. Apply a bivalent or trivalent metal ion solution such as Cu²⁺, Zn²⁺... at a concentration 5 mg/ml in distilled water, at a flow rate of 50 ml/cm²/hour, until saturation.
5. Wash the column with distilled water until the O.D at 280 nm of the eluent falls to the baseline level.
6. Equilibrate the column with the Tris 50mM, AcOH pH 8.0.
7. The obtained metal chelate affinity matrix are ready-to-use.

Applications